Dact1 is expressed during chicken and mouse skeletal myogenesis and modulated in human muscle diseases

Vertebrate skeletal muscle development and repair relies on the precise control of Wnt signaling. Dact1 (Dapper/Frodo) is an important modulator signaling, interacting with key components various transduction pathways. Here, we characterized mRNA protein expression in chicken mouse fetal muscles vivo during differentiation chick primary C2C12 myoblasts vitro . We also performed silico analysis to investigate gene human myopathies, evaluated structure seek explanation for accumulation aggregates nuclei myogenic cells. Our results show first time that both mouse, expressed myogenesis, a strong upregulation as cells engage terminal differentiation, cell cycle withdrawal fusion. In humans, was found be altered specific pathologies, including muscular dystrophies. bioinformatic analyses proteins revealed long intrinsically disordered regions, which may underpin ability interact its many partners addition, has propensity liquid-liquid phase separation, feature explains form nuclear points possible role molecular ‘on’-‘off’ switch. Taken together, our data suggest candidate, multi-faceted regulator amniote myogenesis pathophysiological diseases. • being upregulated differentiation. human, myopathies displays regions transition, features related aggregates.